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Rabbit anti-RPLP0 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to RPLP0

CAS Number

9007-83-4

Synonyms

60S acidic ribosomal protein P0; 60S ribosomal protein L10E

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human RPLP0

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of RPLP0 expression in A549 (A), K562 (B), Jurkat (C), mouse liver (D), mouse testis (E) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of RPLP0 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

6175

Gene ID (Mouse)

11837

Gene ID (Rat)

64205

SwissProt (Human)

P05388

SwissProt (Mouse)

P14869

SwissProt (Rat)

P19945

Available Sizes

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