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Rabbit anti-POLR2C Antibody

Product Specifications

Background

Rabbit polyclonal antibody to POLR2C

CAS Number

9007-83-4

Synonyms

DNA-directed RNA polymerase II subunit RPB3; RNA polymerase II subunit 3; RNA polymerase II subunit B3; DNA-directed RNA polymerase II 33 kDa polypeptide; RPB33; DNA-directed RNA polymerase II subunit C; RPB31

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human POLR2C

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of POLR2C expression in SW620 (A), mouse spleen (B) whole cell lysates.

IHC description

Immunohistochemical analysis of POLR2C staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

5432

SwissProt (Human)

P19387

SwissProt (Mouse)

P97760

Available Sizes

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