Rabbit anti-POLR2C Antibody
Product Specifications
Background
Rabbit polyclonal antibody to POLR2C
CAS Number
9007-83-4
Synonyms
DNA-directed RNA polymerase II subunit RPB3; RNA polymerase II subunit 3; RNA polymerase II subunit B3; DNA-directed RNA polymerase II 33 kDa polypeptide; RPB33; DNA-directed RNA polymerase II subunit C; RPB31
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human POLR2C
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of POLR2C expression in SW620 (A), mouse spleen (B) whole cell lysates.
IHC description
Immunohistochemical analysis of POLR2C staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5432
SwissProt (Human)
P19387
SwissProt (Mouse)
P97760
Available Sizes
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