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Rabbit anti-PDE1B Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PDE1B

CAS Number

9007-83-4

Synonyms

PDE1B1; PDES1B; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B; Cam-PDE 1B; 63 kDa Cam-PDE

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PDE1B

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PDE1B expression in THP1 (A), mouse brain (B), mouse spleen (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PDE1B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5153

Gene ID (Mouse)

18574

Gene ID (Rat)

29691

SwissProt (Human)

Q01064

SwissProt (Mouse)

Q01065

SwissProt (Rat)

Q01066

Available Sizes

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