Rabbit anti-Visfatin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Visfatin
CAS Number
9007-83-4
Synonyms
PBEF; PBEF1; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Visfatin
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Visfatin expression in mouse liver (A), mouse skeletal muscle (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Visfatin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
10135
Gene ID (Mouse)
59027
Gene ID (Rat)
297508
SwissProt (Human)
P43490
SwissProt (Mouse)
Q99KQ4
SwissProt (Rat)
Q80Z29
Available Sizes
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