Rabbit anti-GDF8 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GDF8

CAS Number

9007-83-4

Synonyms

GDF8; Growth/differentiation factor 8; GDF-8; Myostatin

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human GDF8

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GDF8 expression in U937 (A), BT474 (B), mouse heart (C), mouse liver (D), rat skeletal muscle (E) whole cell lysates.

IHC description

Immunohistochemical analysis of GDF8 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.