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Rabbit anti-MBP Antibody

Product Specifications

Background

Rabbit polyclonal antibody to MBP

CAS Number

9007-83-4

Synonyms

Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human MBP

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of MBP expression in SHSY5Y (A), HT29 (B), PC12 (C), mouse brain (D) whole cell lysates.

IHC description

Immunohistochemical analysis of MBP staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

4155

Gene ID (Mouse)

17196

Gene ID (Rat)

24547

SwissProt (Human)

P02686

SwissProt (Mouse)

P04370

SwissProt (Rat)

P02688

Available Sizes

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