Rabbit anti-Monoamine Oxidase B Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Monoamine Oxidase B
CAS Number
9007-83-4
Synonyms
Amine oxidase [flavin-containing] B; Monoamine oxidase type B; MAO-B
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Monoamine Oxidase B
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Monoamine Oxidase B expression in SW480 (A), Hela (B), mouse brain (C), mouse heart (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Monoamine Oxidase B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4129
Gene ID (Mouse)
109731
Gene ID (Rat)
25750
SwissProt (Human)
P27338
SwissProt (Mouse)
Q8BW75
SwissProt (Rat)
P19643
Available Sizes
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