Rabbit anti-EIF6 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to EIF6
CAS Number
9007-83-4
Synonyms
EIF3A; ITGB4BP; Eukaryotic translation initiation factor 6; eIF-6; B (2) GCN homolog; B4 integrin interactor; CAB; p27 (BBP)
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human EIF6
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of EIF6 expression in Hela (A), HepG2 (B), K562 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of EIF6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
3692
Gene ID (Mouse)
16418
Gene ID (Rat)
305506
SwissProt (Human)
P56537
SwissProt (Mouse)
O55135
SwissProt (Rat)
Q3KRD8
Available Sizes
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