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Rabbit anti-EIF6 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to EIF6

CAS Number

9007-83-4

Synonyms

EIF3A; ITGB4BP; Eukaryotic translation initiation factor 6; eIF-6; B (2) GCN homolog; B4 integrin interactor; CAB; p27 (BBP)

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human EIF6

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of EIF6 expression in Hela (A), HepG2 (B), K562 (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of EIF6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

3692

Gene ID (Mouse)

16418

Gene ID (Rat)

305506

SwissProt (Human)

P56537

SwissProt (Mouse)

O55135

SwissProt (Rat)

Q3KRD8

Available Sizes

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