Rabbit anti-EIF3A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to EIF3A
CAS Number
9007-83-4
Synonyms
EIF3S10; KIAA0139; Eukaryotic translation initiation factor 3 subunit A; eIF3a; Eukaryotic translation initiation factor 3 subunit 10; eIF-3-theta; eIF3 p167; eIF3 p180; eIF3 p185
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human EIF3A
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of EIF3A expression in HepG2 (A), Jurkat (B), mouse heart (C), mouse ovary (D) whole cell lysates.
IHC description
Immunohistochemical analysis of EIF3A staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
8661
Gene ID (Mouse)
13669
Gene ID (Rat)
292148
SwissProt (Human)
Q14152
SwissProt (Mouse)
P23116
SwissProt (Rat)
Q1JU68
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