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Rabbit anti-Clathrin Heavy Chain Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Clathrin Heavy Chain

CAS Number

9007-83-4

Synonyms

CLH17; CLTCL2; KIAA0034; Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17; CLH-17

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide of human Clathrin Heavy Chain

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Clathrin Heavy Chain expression in Hela (A), MCF7 (B), mouse lung (C), rat brain (D) whole cell lysates.

IHC description

Immunohistochemical analysis of Clathrin Heavy Chain staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1213

Gene ID (Mouse)

67300

Gene ID (Rat)

54241

SwissProt (Human)

Q00610

SwissProt (Mouse)

Q68FD5

SwissProt (Rat)

P11442

Available Sizes

Frequently Asked Questions

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