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Rabbit anti-CDKN3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CDKN3

CAS Number

9007-83-4

Synonyms

CDI1; CIP2; KAP; Cyclin-dependent kinase inhibitor 3; CDK2-associated dual-specificity phosphatase; Cyclin-dependent kinase interactor 1; Cyclin-dependent kinase-interacting protein 2; Kinase-associated phosphatase

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human CDKN3

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of CDKN3 expression in mouse liver (A) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CDKN3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

1033

Gene ID (Mouse)

72391

Gene ID (Rat)

289993

SwissProt (Human)

Q16667

SwissProt (Mouse)

Q810P3

SwissProt (Rat)

B2RZ50

Available Sizes

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