Rabbit anti-CDKN3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CDKN3
CAS Number
9007-83-4
Synonyms
CDI1; CIP2; KAP; Cyclin-dependent kinase inhibitor 3; CDK2-associated dual-specificity phosphatase; Cyclin-dependent kinase interactor 1; Cyclin-dependent kinase-interacting protein 2; Kinase-associated phosphatase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human CDKN3
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CDKN3 expression in mouse liver (A) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CDKN3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1033
Gene ID (Mouse)
72391
Gene ID (Rat)
289993
SwissProt (Human)
Q16667
SwissProt (Mouse)
Q810P3
SwissProt (Rat)
B2RZ50
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