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Rabbit anti-CD47 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CD47

CAS Number

9007-83-4

Synonyms

MER6; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human CD47

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of CD47 expression in Hela (A) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CD47 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

961

Gene ID (Mouse)

16423

Gene ID (Rat)

29364

SwissProt (Human)

Q08722

SwissProt (Mouse)

Q61735

SwissProt (Rat)

P97829

Available Sizes

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