Rabbit anti-CD47 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CD47
CAS Number
9007-83-4
Synonyms
MER6; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human CD47
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CD47 expression in Hela (A) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CD47 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
961
Gene ID (Mouse)
16423
Gene ID (Rat)
29364
SwissProt (Human)
Q08722
SwissProt (Mouse)
Q61735
SwissProt (Rat)
P97829
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
252821-FITC 100 µL
TNFRSF17 (Tumor Necrosis Factor Receptor Superfamily, Member 17, BCM, BCMA, CD269) (FITC)
Sign In for Pricing
View Details 