Rabbit anti-Ataxin 3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Ataxin 3
CAS Number
9007-83-4
Synonyms
ATX3; MJD; MJD1; SCA3; Ataxin-3; Machado-Joseph disease protein 1; Spinocerebellar ataxia type 3 protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Ataxin 3
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Ataxin 3 expression in Jurkat (A), A549 (B), mouse brain (C), mouse liver (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Ataxin 3 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4287
Gene ID (Mouse)
110616
Gene ID (Rat)
60331
SwissProt (Human)
P54252
SwissProt (Mouse)
Q9CVD2
SwissProt (Rat)
O35815
Available Sizes
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