Anti-Collagen Type IV Antibody

Mouse Monoclonal Antibody specific to Collagen Type IV

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Collagen Type IV Mouse Monoclonal Antibody

Gene Name

COL4A1

NCBI Gene ID

UniProt

P02462

Cellular Locus

Secreted, extracellular space, extracellular matrix, basement membrane

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Collagen Type IV.

Target Antigen

Collagen α-1(IV) chain [Cleaved into: Arresten]

Target

Collagen Type IV

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

IHC

Field of Research

Cancer research

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Tris

Function

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. {UniProtKB:P02463}.; Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. {PubMed:10811134, PubMed:18775695}.

Additionnal Information

<br><br>Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

Human Collagen Type IV. Reactivity with other species has not been investigated.

Formulation

Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.

Buffer pH

pH 7.3-7.7

Target Background

Type IV collagen is the main collagen component of the basement membrane. It is a network- forming collagen that underlies epithelial and endothelial cells and functions as a barrier between tissue compartments. Each type IV collagen molecule is a heterotrimer composed of three alpha chains. Type IV collagen triple helices form open, nonfibrillar networks that associate with laminin assemblies.