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Rabbit anti-EYA3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to EYA3

CAS Number

9007-83-4

Synonyms

Eyes absent homolog 3

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human EYA3

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of EYA3 expression in Hela (A), HT29 (B), mouse eye (C), mouse testis (D), rat brain (E), rat liver (F) whole cell lysates.

IHC description

Immunohistochemical analysis of EYA3 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

2140

Gene ID (Mouse)

14050

SwissProt (Human)

Q99504

SwissProt (Mouse)

P97480

Available Sizes

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