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Rabbit anti-BTEB1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to BTEB1

CAS Number

9007-83-4

Synonyms

BTEB; BTEB1; Krueppel-like factor 9; Basic transcription element-binding protein 1; BTE-binding protein 1; GC-box-binding protein 1; Transcription factor BTEB1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human BTEB1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of BTEB1 expression in mouse lung (A), mouse liver (B), rat brain (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of BTEB1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

687

Gene ID (Rat)

117560

SwissProt (Human)

Q13886

SwissProt (Mouse)

O35739

SwissProt (Rat)

Q01713

Available Sizes

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