Rabbit anti-eIF3H Antibody
Product Specifications
Background
Rabbit polyclonal antibody to eIF3H
CAS Number
9007-83-4
Synonyms
EIF3S3; Eukaryotic translation initiation factor 3 subunit H; eIF3h; Eukaryotic translation initiation factor 3 subunit 3; eIF-3-gamma; eIF3 p40 subunit
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human eIF3H
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of eIF3H expression in HL60 (A), HT29 (B), NIH3T3 (C), mouse testis (D), rat spleen (E) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of eIF3H staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
8667
Gene ID (Mouse)
68135
Gene ID (Rat)
100911110; 299899
SwissProt (Human)
O15372
SwissProt (Mouse)
Q91WK2
SwissProt (Rat)
Q6P9U8
Available Sizes
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