Rabbit anti-DARS Antibody
Product Specifications
Background
Rabbit polyclonal antibody to DARS
CAS Number
9007-83-4
Synonyms
Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human DARS
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of DARS expression in MCF7 (A), SW480 (B), mouse brain (C), rat liver (D) whole cell lysates.
IHC description
Immunohistochemical analysis of DARS staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1615
Gene ID (Mouse)
226414
Gene ID (Rat)
116483
SwissProt (Human)
P14868
SwissProt (Mouse)
Q922B2
SwissProt (Rat)
P15178
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