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Rabbit anti-PGM1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PGM1

CAS Number

9007-83-4

Synonyms

Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PGM1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PGM1 expression in HepG2 (A), mouse heart (B), rat liver (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PGM1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5236

Gene ID (Mouse)

72157

SwissProt (Human)

P36871

SwissProt (Mouse)

Q9D0F9

SwissProt (Rat)

P38652

Available Sizes

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