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Rabbit anti-APRT Antibody

Product Specifications

Background

Rabbit polyclonal antibody to APRT

CAS Number

9007-83-4

Synonyms

Adenine phosphoribosyltransferase; APRT

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human APRT

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of APRT expression in THP1 (A), A549 (B), K562 (C), mouse intestine (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of APRT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

353

Gene ID (Mouse)

11821

Gene ID (Rat)

292072

SwissProt (Human)

P07741

SwissProt (Mouse)

P08030

SwissProt (Rat)

P36972

Available Sizes

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