Rabbit anti-TIM Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TIM
CAS Number
9007-83-4
Synonyms
TPI; Triosephosphate isomerase; TIM; Triose-phosphate isomerase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human TIM
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of TIM expression in Jurkat (A), HepG2 (B), mouse kidney (C), mouse brain (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of TIM staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
7167
Gene ID (Mouse)
21991
Gene ID (Rat)
24849
SwissProt (Human)
P60174
SwissProt (Mouse)
P17751
SwissProt (Rat)
P48500
Available Sizes
Frequently Asked Questions
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