Rabbit anti-CapZ alpha-2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CapZ alpha-2
CAS Number
9007-83-4
Synonyms
F-actin-capping protein subunit alpha-2; CapZ alpha-2
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human CapZ alpha-2
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CapZ alpha-2 expression in Hela (A), THP1 (B), mouse brain (C), mouse heart (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CapZ alpha-2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
830
Gene ID (Mouse)
12343
Gene ID (Rat)
493810
SwissProt (Human)
P47755
SwissProt (Mouse)
P47754
SwissProt (Rat)
Q3T1K5
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