Rabbit anti-Histone H2B Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Histone H2B
CAS Number
9007-83-4
Synonyms
H2BFQ; Histone H2B type 2-E; Histone H2B-GL105; Histone H2B.q; H2B/q
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Histone H2B
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Histone H2B expression in SW480 (A), BT474 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Histone H2B staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
8349
Gene ID (Mouse)
319190
SwissProt (Human)
Q16778
SwissProt (Mouse)
Q64524
Available Sizes
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