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Rabbit anti-Histone H2B Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Histone H2B

CAS Number

9007-83-4

Synonyms

H2BFQ; Histone H2B type 2-E; Histone H2B-GL105; Histone H2B.q; H2B/q

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Histone H2B

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Histone H2B expression in SW480 (A), BT474 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of Histone H2B staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

8349

Gene ID (Mouse)

319190

SwissProt (Human)

Q16778

SwissProt (Mouse)

Q64524

Available Sizes

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