Rabbit anti-TCF4 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TCF4
CAS Number
9007-83-4
Synonyms
BHLHB19; ITF2; SEF2; Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human TCF4
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of TCF4 expression in HepG2 (A), MCF7 (B), mouse brain (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of TCF4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
6925
Gene ID (Mouse)
21413
Gene ID (Rat)
84382
SwissProt (Human)
P15884
SwissProt (Mouse)
Q60722
SwissProt (Rat)
Q62655
Available Sizes
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