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Rabbit anti-TCF4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to TCF4

CAS Number

9007-83-4

Synonyms

BHLHB19; ITF2; SEF2; Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human TCF4

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of TCF4 expression in HepG2 (A), MCF7 (B), mouse brain (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of TCF4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

6925

Gene ID (Mouse)

21413

Gene ID (Rat)

84382

SwissProt (Human)

P15884

SwissProt (Mouse)

Q60722

SwissProt (Rat)

Q62655

Available Sizes

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