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Rabbit anti-Emerin Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Emerin

CAS Number

9007-83-4

Synonyms

EDMD; STA; Emerin

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Emerin

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Emerin expression in Hela (A), Jurkat (B), PC3 (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Emerin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

2010

Gene ID (Mouse)

13726

Gene ID (Rat)

25437

SwissProt (Human)

P50402

SwissProt (Mouse)

O08579

SwissProt (Rat)

Q63190

Available Sizes

Frequently Asked Questions

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