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Rabbit anti-Hexokinase 2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Hexokinase 2

CAS Number

9007-83-4

Synonyms

Hexokinase-2; Hexokinase type II; HK II; Muscle form hexokinase

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Hexokinase 2

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Hexokinase 2 expression in SHSY5Y (A), MCF7 (B), K562 (C), COS7 (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Hexokinase 2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

3099

Gene ID (Mouse)

15277

Gene ID (Rat)

25059

SwissProt (Human)

P52789

SwissProt (Mouse)

O08528

SwissProt (Rat)

P27881

Available Sizes

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