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Rabbit anti-ATF6 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ATF6

CAS Number

9007-83-4

Synonyms

Cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha; Activating transcription factor 6 alpha; ATF6-alpha

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide of human ATF6

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ATF6 expression in K562 (A), PC3 (B), mouse heart (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of ATF6 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

22926

Gene ID (Mouse)

226641

Gene ID (Rat)

304962

SwissProt (Human)

P18850

SwissProt (Mouse)

F6VAN0

SwissProt (Rat)

G3V909

Available Sizes

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