Rabbit anti-HIF1 beta Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HIF1 beta
CAS Number
9007-83-4
Synonyms
BHLHE2; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant protein corresponding to human HIF1 beta.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/200 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HIF1 beta expression in MCF7 (A), NIH3T3 (B), rat brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of HIF1 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
405
SwissProt (Human)
P27540
Available Sizes
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