Rabbit anti-Beta3-tubulin Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Beta3-tubulin

CAS Number

9007-83-4

Synonyms

TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Beta3-tubulin. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/2000 - 1/5000), IH (1/200 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Beta3-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Beta3-tubulin staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.