Rabbit anti-AMPK alpha 1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to AMPK alpha 1
CAS Number
9007-83-4
Synonyms
AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant protein corresponding to human AMPK alpha 1.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of AMPK alpha 1 expression in Hela (A), NIH3T3 (B), PC12 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of AMPK alpha 1 staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5562
SwissProt (Human)
Q13131
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