Mouse anti-XRCC4 Antibody

Product Specifications

Background

Mouse monoclonal antibody to XRCC4

CAS Number

9007-83-4

Synonyms

DNA repair protein XRCC4; X-ray repair cross-complementing protein 4

Host

Mouse

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human XRCC4. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IP

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/200 - 1/500), IP (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of XRCC4 expression in Hela (A), 293T (B) whole cell lysates.

IHC description

Immunohistochemical analysis of XRCC4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.