Mouse anti-TNF alpha Antibody

Product Specifications

Background

Mouse monoclonal antibody to TNF alpha

CAS Number

9007-83-4

Synonyms

TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Host

Mouse

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human TNF alpha. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of TNF alpha expression in recombinant human TNF-a protein (A) whole cell lysates.

IHC description

Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.