Mouse anti-Swiprosin-2 Antibody
Product Specifications
Background
Mouse monoclonal antibody to Swiprosin-2
CAS Number
9007-83-4
Synonyms
SWS2; EF-hand domain-containing protein D1; EF-hand domain-containing protein 1; Swiprosin-2
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Swiprosin-2. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IF/IC
Concentration
1mg/mL
Dilution
WB (1/1000 - 1/2000), IF/IC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Swiprosin-2 expression in mouse spleen (A), rat spleen (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Swiprosin-2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
80303
Gene ID (Mouse)
98363
SwissProt (Human)
Q9BUP0
SwissProt (Mouse)
Q9D4J1
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