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Mouse anti-STAT3 Antibody

Product Specifications

Background

Mouse monoclonal antibody to STAT3

CAS Number

9007-83-4

Synonyms

APRF; Signal transducer and activator of transcription 3; Acute-phase response factor

Host

Mouse

Reactivity

H, M, R

Immunogen

Recombinant protein corresponding to human STAT3.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of STAT3 expression in Hela (A), mouse heart (B), rat heart (C) whole cell lysates.

IHC description

Immunohistochemical analysis of STAT3 staining in human colon cancer, mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

6774

SwissProt (Human)

P40763

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