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Mouse anti-SMAD3 Antibody

Product Specifications

Background

Mouse monoclonal antibody to SMAD3

CAS Number

9007-83-4

Synonyms

MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Host

Mouse

Reactivity

H, M, R

Immunogen

Recombinant protein corresponding to human SMAD3.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of SMAD3 expression in 293T (A), Jurkat (B), mouse heart (C), rat heart (D) whole cell lysates.

IHC description

Immunohistochemical analysis of SMAD3 staining in human liver, human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

4088

SwissProt (Human)

P84022

Available Sizes

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