Mouse anti-PCNA Antibody

Product Specifications

Background

Mouse monoclonal antibody to PCNA

CAS Number

9007-83-4

Synonyms

Proliferating cell nuclear antigen; PCNA; Cyclin

Host

Mouse

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human PCNA. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/2000 - 1/5000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PCNA expression in Hela (A), 293T (B), NIH3T3 (C), rat brain (D) whole cell lysates.

IHC description

Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.