What is the catalog number for Mouse anti-p53 Antibody?

The catalog number for Mouse anti-p53 Antibody is DL95540A.

What sizes are available for Mouse anti-p53 Antibody?

Mouse anti-p53 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Mouse anti-p53 Antibody come in?

Mouse anti-p53 Antibody is available in 2 different pack sizes.

Is Mouse anti-p53 Antibody in stock?

Mouse anti-p53 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Mouse anti-p53 Antibody

Product Specifications

Background

Mouse monoclonal antibody to p53

CAS Number

9007-83-4

Synonyms

P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Host

Mouse

Reactivity

H

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human p53. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IF/IC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of p53 expression in 293T (A) whole cell lysates.

IHC description

Immunohistochemical analysis of p53 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of p53 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .