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Rabbit anti-Caldesmon Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Caldesmon

CAS Number

9007-83-4

Synonyms

CAD; CDM; Caldesmon; CDM

Host

Rabbit

Reactivity

H, M, R, C

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Caldesmon. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Caldesmon expression in HepG2 (A), Hela (B), NIH3T3 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Caldesmon staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

800

Gene ID (Rat)

25687

SwissProt (Human)

Q05682

SwissProt (Rat)

Q62736

Available Sizes

Frequently Asked Questions

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