Rabbit anti-Villin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Villin
CAS Number
9007-83-4
Synonyms
VIL; Villin-1
Host
Rabbit
Reactivity
H, M, B, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Villin. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Villin expression in K562 (A), HT29 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Villin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7429
Gene ID (Mouse)
22349
SwissProt (Human)
P09327
SwissProt (Mouse)
Q62468
Available Sizes
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