Rabbit anti-HER2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HER2
CAS Number
9007-83-4
Synonyms
HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340
Host
Rabbit
Reactivity
H, M, R, D
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HER2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HER2 expression in PC3 (A), MCF7 (B), NIH3T3 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of HER2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
2064
Gene ID (Mouse)
13866
SwissProt (Human)
P04626
SwissProt (Mouse)
P70424
SwissProt (Rat)
P06494
Available Sizes
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