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Rabbit anti-HSD3B7 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to HSD3B7

CAS Number

9007-83-4

Synonyms

3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta (5) -C27 steroid oxidoreductase; C (27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSD3B7. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of HSD3B7 expression in Lovo (A), MCF7 (B), Hela (C) whole cell lysates.

IHC description

Immunohistochemical analysis of HSD3B7 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

80270

Gene ID (Mouse)

101502

Gene ID (Rat)

246211

SwissProt (Human)

Q9H2F3

SwissProt (Mouse)

Q9EQC1

SwissProt (Rat)

O35048

Available Sizes

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