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Rabbit anti-PLC beta 3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PLC beta 3

CAS Number

9007-83-4

Synonyms

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PLC beta 3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PLC beta 3 expression in SHSY5Y (A), Hela (B), NIH3T3 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of PLC beta 3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

5331

SwissProt (Human)

Q01970

SwissProt (Mouse)

P51432

SwissProt (Rat)

Q99JE6

Available Sizes

Frequently Asked Questions

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