Rabbit anti-CRK (pY221) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CRK (pY221)
CAS Number
9007-83-4
Synonyms
Adapter molecule crk; Proto-oncogene c-Crk; p38
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CRK (pY221) expression in Hela (A), THP1 (B), mouse liver (C), rat liver (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CRK (pY221) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1398
Gene ID (Mouse)
12928
Gene ID (Rat)
54245
SwissProt (Human)
P46108
SwissProt (Mouse)
Q64010
SwissProt (Rat)
Q63768
Available Sizes
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