Rabbit anti-GPR27 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GPR27
CAS Number
9007-83-4
Synonyms
SREB1; Probable G-protein coupled receptor 27; Super conserved receptor expressed in brain 1
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR27. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GPR27 expression in HEK293T (A), SGC7901 (B), mouse lung (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of GPR27 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2850
Gene ID (Mouse)
14761
Gene ID (Rat)
65275
SwissProt (Human)
Q9NS67
SwissProt (Mouse)
O54897
SwissProt (Rat)
Q9JJH3
Available Sizes
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