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Rabbit anti-EIF2AK1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to EIF2AK1

CAS Number

9007-83-4

Synonyms

HRI; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinase

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EIF2AK1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of EIF2AK1 expression in K562 (A), LO2 (B), PC12 (C), AML12 (D) whole cell lysates.

IHC description

Immunohistochemical analysis of EIF2AK1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

27102

Gene ID (Mouse)

15467

Gene ID (Rat)

27137

SwissProt (Human)

Q9BQI3

SwissProt (Mouse)

Q9Z2R9

SwissProt (Rat)

Q63185

Available Sizes

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