Rabbit anti-GPRC5D Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GPRC5D
CAS Number
9007-83-4
Synonyms
G-protein coupled receptor family C group 5 member D
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPRC5D. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GPRC5D expression in MCF7 (A), SGC7901 (B), Panc1 (C), CT26 (D), PC12 (E) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of GPRC5D staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
55507
Gene ID (Mouse)
93746
SwissProt (Human)
Q9NZD1
SwissProt (Mouse)
Q9JIL6
Available Sizes
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