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Rabbit anti-GPRC5D Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GPRC5D

CAS Number

9007-83-4

Synonyms

G-protein coupled receptor family C group 5 member D

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPRC5D. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GPRC5D expression in MCF7 (A), SGC7901 (B), Panc1 (C), CT26 (D), PC12 (E) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of GPRC5D staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

55507

Gene ID (Mouse)

93746

SwissProt (Human)

Q9NZD1

SwissProt (Mouse)

Q9JIL6

Available Sizes

Frequently Asked Questions

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